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A certificate of analysis (COA) is the record of the tests run on one production lot. Before a vial is used, it is worth spending two minutes with that document. Start with the lot number The lot number printed on the…
High-performance liquid chromatography (HPLC) pushes a dissolved sample through a packed column. Different molecules travel at different speeds and leave the column at different times, where a UV detector records them…
Mass spectrometry (MS) ionises a sample and measures the mass-to-charge ratio of the resulting ions. For a peptide, the observed mass is compared with the theoretical mass of the expected sequence. Why it matters Two…
Endotoxins are lipopolysaccharide fragments from the outer membrane of gram-negative bacteria. They are stable, hard to remove and active at very low concentrations. The LAL test The Limulus Amebocyte Lysate (LAL)…
Lyophilized (freeze-dried) peptides are shipped as a dry powder because it is far more stable than a solution. Before opening Keep sealed vials cold, dry and away from light. Many laboratories store them at −20 °C for…
A lot is a quantity of material made in one production run. Every vial from that run carries the same lot number. Why it matters The lot number connects a vial to its batch record and its certificate of analysis. If a…
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